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Family Leguminosae
Paso-paso
Dioclea reflexa Hook. f.
SEA BEAN
Chinese

Scientific names Common names
Dioclea labathei H.St.John. Paso-paso (Tag.)
Dioclea reflexa Hook.f. Masupaso (Tag.)
Lepidamphora volubilis Zoll. Drift seed (Engl.)
  Horse eye Engl.)
  Marble vine (Engl.)
  Sea bean (Engl.)
  Sea purse (Engl.)
Dioclea reflexa Hook.f. is an accepted name. The Plant List

Other vernacular names
LINGALA: Bonkele.
COUNTRY: Name
COUNTRY: Name
NIGERIA: Agba-arin, Ukpo, Ebba.
PORTUGUESE: Corda iple.

Gen info
- The Dioclea clade comprises four genera and approximately 60 species of the tribe Diocleae: Cleobulia (4 species), Cymbosema (1), Dioclea (ca. 50), Luzoni (1), and Micropsychanthus (3-4). (20)
- Dioclea is a genus of flowering plants in the pea family, Fabaceae, which is native to the Americas. The seeds of these legumes are buoyant drift seeds, dispersed by ocean and river currents.

Botany
Dioclea reflexa is an attractive, vigorous, climbing woody vine. Stems 4 cm in diameter, older stems up to 5 cm thick. Branches terete, at first pubescent. Leaflets subcoriaceous, acute or obtuse, hairy beneath, 10-15 cm long. Mature pods are 10-20 cm long, about 5 cm wide, and about 2 cm thick, containing 3-4 hard biconvex seeds, 25-30 mm in greatest diameter; brick, brownish-red, dark brown to black, sometimes with dark speckles.

Distribution
- Pantropical sea-drifted.
- Widely distributed within tropical Africa and South Africa.
- Found in coastal forests along tropical seashores worldwide.
- Seeds distributed by ocean current. Often found washed in seashores.

-
Constituents
- Study of ethyl acetate soluble fraction of roots isolated two new flavonoids, dioclins A (1) and G (2) along with 3,5-dihydroxy-4-methoxybenzoic acid (3), lupeol (4) and the rare dipeptide, auratiamide acetate (5). (see study below) (4)
- Proximate analysis of D. reflexa seeds yielded ash (9.17±0.01%), crude fat (10.65±0.01%). fiber (9.25±0.03%). moisture (13.30±0.01%), crude protein (26.00±0.43%), carbohydrate (31.63±0.12%) and calorific value (1372.07 kj/100g). Mineral analysis yielded in mg/L potassium (4.09±0.04). sodium (4.88±0.25), magnesium (0.80±0.042), zinc (2.10±0.03), iron (0.48±0.05), manganese (0.37±0.61) and calcium (7.11±0.42). Phytochemical screening of the seeds yielded alkaloids, cardiac glycosides, phlobatannin and saponin. (6)
- Study of whole seed for proximate composition and oil characteristics yielded total ash 2.51%, moisture 7.2%, crude fiber 2.0%, crude fat 7.46%, crude protein 13.00%, carbohydrate 28.02%, and energy value of 370.02 Kcal. Fatty acid composition of seed oil yielded major constituents of linoleic acid 28.95, oleic 22.79, palmitic 12.88, stearic 7.45, behenic 4.29, and lignoceric acid 2.69, and lesser amounts of myristic, pentadecanoic, heptadecanoic, arachidic, and hexacosanoic acid. (9)
- Hydrodistillation, GC, and GC-MS studies of D. reflexa roots for volatile oils yielded a total of 15 compounds. Hydrocarbons were the dominant group representing 69.27%. Oxygenated monoterpenoids identified included terpinen-4-ol (2.47%), myrtenal (1.69%), verbenone (1.25%), trans-pinocarveol (2.86%), and thymol (1.30%). Stearyl iodide 10.33%) was also identified. (12)
- Gas chromatography of seed oil for fatty acid composition showed individual unsaturated fatty acid to be oleic acid at 18:1 (0.8%), while saturated fatty acids were palmitic acid 16:0 18:0 ( 21.9%(10.2%), stearic acid 18:0 (21.9%). (15)
- Study of ethanol soluble part of roots isolated reflevone (1), a new flavone, together with mearnsetin (2), 7,4'-dihydroxyflavone (3), and two known phytosterols, ß-sitosterol (4), and stigmasterol (5).(see study below) (18)
- Study of methanol extract of seeds yielded two steroids, namely: taraxasterol and stigmasterol. (23)

Properties
- Studies have suggested antioxidant, antidiabetic, antimicrobial, neuroprotective, nephroprotective, immunosuppressant, anticancer, hepatoprotective properties.

Parts used
Leaves, seeds.

Uses

Edibility
- In Nigeria, flour prepared from the seeds used as a soup thickener. (2)
- Seeds used as emergency food in times of need.
- Cake and seed flour has appreciable amounts of protein with potential as human food.
Folkloric
- No reported folkloric medicinal use in the Philippines.
- In Nigeria, leaves of D. reflexa and Vernonia amygdalina in coconut oil applied to furuncles. Powdered preparation of D. reflexa with other herbs, salt and potash applied for pruritus. (14) A spoonful of powdered fruit mixed with rhizome of Aframomum melegueta in palm kernel oil taken for stomach ulcer. (17) A novel medication compounded with Dioclea reflexa, Cocos nucifera, and Piper guainenses has been registered with the NAFDAC in Nigeria for the treatment of cancer presented at early stages.   (19)
- Decoction or poultice of leaves applied topically to treat aches and pains, skin rashes and sores. (21)
Others
- Oil: Due to high saponification and iodine levels in the oil, it has found applications in alkyl resin, shoe polish, liquid soap, and shampoo production.
(15)
- Crafts: Large attractive seeds used for making jewelry.(•) Seeds used as marbles in a traditional Nigerian game. (21)
- Gum: Seed yields a gum with potential as pharmaceutical excipient. (see study below) (2)
- Insecticide: In Africa, seeds uses as insecticide and arachnicide. (21)

Studies
Potential as Excipient / Gum from Seeds:
Study reports on the extraction of D. reflexa gum (DR0gum) from seeds, which was modified by ionotropic gelation in calcium chloride solution. The basic similarities and differences in the physicochemical and functional properties of DR-gum, SXR-gum and guar gum indicated potential as pharmaceutical excipient. (2)
Antinutritional Properties / Effect of Roasting: The presence of some antinutritional constituents such as trypsin inhibitors, phytic acid, tannins, hemagglutinins can cause adverse physiological effects and diminish the availability of nutrient, which may interfere with digestion of carbohydrates and proteins. Anti-nutritional properties can be reduced by dehulling. soaking. boiling, germination and roasting operations. Roasting can improve flavor, texture, and acceptability of products. Phytochemical constituents (phenols, flavonoids, cardoac glycosides, sterols, etc) are influenced by seed variety (light brown, dark brown, black) and roasting conditions. Optimized roasting conditions can be used to produce foods with potentials to promote better health. (3)
Flavonoids Dioclins A and B / Antioxidants / Roots: Study of ethyl acetate soluble fraction of roots isolated two new flavonoids, dioclins A (1) and B (2) along with 3,5-dihydroxy-4-methoxybenzoic acid *3), lupeol (4) and the rare dipeptide, auratiamide acetate (5). Compounds 1 and 2 showed significant antioxidant activity in DPPH radical scavenging assay. (4)
Antidiabetic / Mature Seeds: Study evaluated the antidiabetic effect pf seed flour of D. reflexa on alloxan-induced diabetic rats. The seed flour was found to be more effective than the standard drugs gliclazide and metformin. The oral hypoglycemic effect was probably mediated via increased peripheral utilization of glucose. (5)
Phytoconstituents and Nutrient Potential: Study showed Dioclea reflexa seeds contain alkaloids and glycosides of pharmacological significance and high levels of carbohydrate, crude protein and moisture content with potential as supplementary source of essential nutrients for man and livestock. (6)
Attenuation of Cognitive Impairment in Insulin-Dependent DM / Formulated Meal of Marble Vine and Plantain: Study evaluated the protective effect of formulated marbler vine/plantain dough meals on cognitive impairment in diabetic Wistar rats riven a high fat diet and a single dose of intraperitoneal STZ. Weight gain/loss, glucose and insulin levels, oxidative damage, neurological dysfunction, and histological alterations were assessed. The diet significantly ameliorated the neurological dysfunction as evidenced by increased dioamine concentration and lowered acetylcholinesterase activity, further supported by brain histopathological study. Results suggest the formulated dough has neuroprotective potentials potentials in preventing neurological compiications arising from diabetes. (7)
Antimicrobial / Leaves: Study evaluated the antimicrobial activity of crude ethanolic, methanolic, and acetonic leaf extracts from four medicinal plants against nine microorganisms viz. six bacteria, S. aureus, P. mirabilis, K. pneumonia, S. typhi, S. pneumonia, E. coli, and three fungi, A. flavus, C. albicans, and F. solani. Extracts of Dioclea reflexa showed highest broad spectrum antibacterial activity. (8)
Dioclimidazole / Anticholinesterase / Antibacterial / Seeds: Study isolated a new secondary metabolites, dioclimidazole (1) from the chloroform extract of seeds of Dioclea reflexa. The compound was compared with huperzine A in an anticholinesterase assay. Dioclimidazole showed significant anticholinesterase activity in vitro. It also showed appreciable antibacterial activity against E. coli and B. subtilis. (10)
Effect on Hematological and Renal Parameters After CCl4 Challenges / Seeds: Study evaluated the effect of methanol extract of D. reflexa seed on male rats following acute (single) and repeated dose (chronic) carbon tetrachloride intoxication in male albino rats. Results showed the seeds possess capacity to boost hematological parameters and protect the kidney from acute and chronic toxicological challenges. (11)
Reflevone / Radical Scavenging / Immunosuppressant / Urease Inhibitory / Anticancer / Roots: Study of roots isolated a new flavonoid, reflorvone. The compound showed very strong immunosuppressant effect on phytohemagglutiinin (PHA) stimulated T-cell, good urease inhibitory activity compared to standard urea, and strong free radical scavenging activity. It also showed mild cytotoxic effect on human lung cancer cell line (NHI-H460). (13)
Hepatoprotective / Antioxidant / Seed: Study showed a methanolic extract of seed following acute and chronic carbon tetrachloride intoxication in male albino rats. In both models, levels of transaminases and malondialdehyde were significantly (p<0.05) reduced in extract treated rats. There was also boosting in the levels of superoxide dismutase and catalase activities. Results suggest strong antioxidant and hepatoprotective properties in both acute and chronic hepatocellular damage and oxidative stress. (16)
Flavonoids / Anticancer / Roots: Study of ethanol soluble part of roots isolated reflevone (1), a new flavone, together with mearnsetin (2), 7,4'-dihydroxyflavone (3), and two known phytosterols, ß-sitosterol (4), and stigmasterol (5). The crude roots extract showed significant activity against prostate cancer cell line with IC50 of 5.18 µg/mL. (18)
Flavonoid and Phenolic Content / Antibacterial / Leaves: Phytochemical screening of leaves showed presence of terpenes only in the n-hexane extract while the methanol extract yielded flavonoids, steroids and terpenoids. Alkaloids, saponins, and anthraquinones wer3e absent in all the extracts. The methanol extract was active against K. pneumonia and P aeruginosa, and inactive against E coli, P. vulgaris, and S. aureus. Total phenolic content was 41.58 mg GE equivalent / g extract while total flavonoid was 29.82 QE / g extract. (22)
Protein Source / Digestibility / Seeds: Study evaluated the potential of three common and under-utilized tropical leguminous seeds i.e., Dioclea reflexa, Tomentosa nilotica, and Monodora myristica as supplementary feed to ruminant livestock. The study focused on the effect of precessing methods on the nutritional composition (proximate, fiber fractions, minerals and nutrients) and in vitro digestibility of the seeds, and and suitability as feedstuff (protein sources) in small ruminant feed, especially during off-season. Dioclea reflexa showed highest organic matter digestibility, short-chain fatty acids, metabolizable energy and in vitro dry matter digestibility values. Roasting improved the nutritive value. Results suggest Dioclea reflexa can be incorporated in ruminant diet as protein source, especially for off-season. (24)
Seed Gum Potential as Suspending Agent: Study evaluated the potential of D. reflexa seed gum as suspending agent in metronidazole suspension. Tragacanth gum (TR) was used as reference. Results showed the viscosity of DR gum was slightly higher than TR. Sedimentation rate showed no significant difference. The DR gum compared favorably with TR, indicating good properties as potential suspending agent in MTZ suspensions. (25)

Availability
Wild-crafted.

August 2021

PHOTOS / ILLUSTRATIONS
IMAGE SOURCE: Photograph / Dioclea reflexa / Olivier Gaubert / Jann 23, 2015 / click on image to go to source page / / Floredeguyane.piwigo.com
OTHER IMAGE SOURCE: /Gliricidia sepium (Jacq.) Kunth ex Walp. - quickstick GLSE2 / Steve Hurst @ USDA-NRCS PLANTS Database / USDA
 
IMAGE SOURCE: / Photos (2) / Pterocaulon redolens (Willd.) F.-Vill. / © Collected by www.plant.ac.cn / ZHIWUTONG / CLICK ON IMAGE TO GO TO SOURCE PAGE
IMAGE SOURCE: / Line drawing / Pterocaulon redolens (Willd.) F.-Vill. / © Collected by www.plant.ac.cn / ZHIWUTONG / CLICK ON IMAGE TO GO TO SOURCE PAGE
OTHER IMAGE SOURCE: / Photo / Rutaceae : Lunasia amara det. John Rey Callado / Leaf / Copyright © 2012 by P.B. Pelser & J.F. Barcelona (contact: pieter.pelser@canterbury.ac.nz) [ref. DOL52015] / Non-Commercial Use / Phytoimages.siu.edu
OTHER IMAGE SOURCE: / Flower close-up / dracobotanicus -- Wayne Dumbleton / Creative Commons Attribution / flickr / Click on graphic to see original image / flickr /
OTHER IMAGE SOURCE: / Illustration / Cissampelos pareira L. [5809-247450-161657] / Indian medicinal plants, vol. 1: t. 42 / PlantIllustrations.org
flickr /
Pacific Island Ecosystems at Risk (PIER)
Photos ©Godofredo Stuart / StuartXchange
Content © Godofredo Stuart / StuartXchange
Content / Photos © Godofredo Stuart / StuartXchange
Photos © Godofredo Stuart / StuartXchange

Additional Sources and Suggested Readings
(1)
Notes on Malesian Fabaceae (Leguminosae-Papilionoideae): The genera Dioclea, Luzonia, and Macropsychanthus / Frits Adema / Biodiversity, Evolution and Biogeography of Plants, 1998
(2)
Intrinsic and Functional Properties of a Gelling Gum from Dioclea reflexa: A Potential Pharmaceutical Excipient / Philip F Builders, Chukwuemela C Mbah, Anthony A Attama / Journal of Pharmaceutical Research International, 2012:pp 50-68 / DOIL 10.9734/BJPR/2012/071
(3)
Effect pf Roasting on the Phytochemical Properties of Three Varieties of Marble Vine Dioclea reflexa Using Response Surface Methodology / Mary A Ajstta, Stephen A Akinola, Oluwatooyin F Osundahunsi / Preventi ve Nutrition and Food Science, Dec 2019; 24(4): pp 468-477 / PMID:31915643 /
DOI: 10.3746/pnf.2019.24.4.468
(4)
Dioclins A and B, new antioxidant flavonoids from Dioclea reflexa / Abdulkabir Oladele Oladimeji, Ibrahim Adebayo Oladosu, Muhammad Shaiq Ali, and Mehreen Lateef / Natural Products Research, 2018; 32(17) / DOI: heeps://doi.org/10.1080/14786419.2017.1361949

(5)
Exploration of the anti-diabetic effect of the mature seeds of Dioclea reflexa / Adepoju Adeyinka GK, Bologun O M / Journal of Medicinal Plants Research, June 2014; 8(22): pp 811-813 /
DOI: https://doi.org/10.5897/JMPR10.608
(6)
Oil Content and Fatty Acid Composition of Dioclea reflexa Seeds / Ibironke A Ajayi / IOSR-Journal of Applied Chemistry, July 2014; 7(7): pp d68-73 / eISSN: 2278-5736
(7)
Cognitive impairment by non-insulin dependent diabetes mellitus was attenuated by dietary supplements of marbgle vine (Dioclea reflexa) and plantain (Musa paradisiaca) dough meals in albino rats / Mary A Ajatta, Olumayoma R Oladipupo, Sunday S Josiah et al / Journal of Food Biochemistry, 45(3) / e13473
(8)
Antimicrobial Efficacy of the Leaves of Bioclea reflexa, Mucuna pruriens, Ficus asperifolia, and Tragia
spathulata
/ A O Ogundare and O B Oluranfemi / Research Journal of Microbiology, 2007; 2: pp 392-396 /
DOI: https://doi.org/10.3923/jm.2007.392.396
(9)
Physicochemical Composition and Oil Characterization of Dioclea reflexa and Monodora myristica Seeds / Akinyede A J, Fagbemi T N, Osundahunsi O F and Aluko E F / Applied Tropical Agriculture, 2016; 21(3): pp 8-11
(10)
Bioactive of Dioclimidazole from Dioclea reflexa Seeds / I A Oladosu, J O Echeme, and M F Zubair / Middle East Journal of Scientific Research, 2010; 6(6): pp 575-579 / ISSN: 1990-9233
(11)
Effect of Methanolic Extract of Dioclea reflexa Hook F Seed on some Hematological and Kidney Function Parameters in Rats following Single or Repeated Carbon Tetrachloride Intoxication / Sunday Ene-Ojo Atawodi and Uju Dorothy IIiemene / British Biotechnology Journal, 2014; 4(12): pp 1253-1262 / ISSN: 2231-2927
(12)
Volatile constituent of sea purse, Dioclea reflexa root / Oladosu Ibrahim A and Oladimeji Abdulkabir O / Elixir Organic Chemistry, 2012; 45: pp 7738-7740
(13)
Determination of In-Vitro Biochemical Activities of Reflevone, A New Natural Organic Compound / Abdulkabir Adebayo Oladele Oladimeji, Ibrahim Adebayo Oladosu, Ajmal Khan / Journal of Biologically Active Products from Nature / 2017; 7(6) / DOI: https:..doi.org/10.1080/22311866.2017.1409135
(14)
Ethnobotanical Survey of Plants Used in Treatment of Inflammatory Diseases in Ogun State of Nigeria / Omonike O Ogbole, Adebayo A Gbolade, Edith O Ajaiyeoba / European Journal of Scientific Research, 2010; 43(2): pp 183-191 / ISSN: 1450-216X
(15)
Extraction and Characterization of Dioclea reflexa Hook.f. Seed Oil / Faleye Francis Jide / Pakistan Journal of Scientific and Industrial Research, 2010; 53(2): pp 72-75
(16)
In vivo antioxidant and hepatoprotective effects of methanolic extract of Dioclea reflexa seed in rats following acute or chronic liver injury / Uju Dorothy Iliemene, Sunday Ene-Pjp Atawodi / Bangladesh Journal of Pharmacology, 2014; 9(1) / DOI: 10.3329/bjp.v9i1.17452
(17)
Ethnobiological survey of traditional medicine practice for Gastrointestinal tract infections in Oyo State, Nigeria / Borokini T I, Clement M, Dickson N J, and Edagbo D E / Topclass Journal of Herbal Medicine, June 2013; 2(6): pp 131-139 / ISSN: 2315-8840
(18)
Flavonoids from the roots of Dioclea reflexa Hook.f. / A O Oladimeji, I A Oladosu, M S Ali, Z Ahmed / Bulletin of the Chemical Society of Ethiopia, 2015; 29(3) / DOI: 10.4314/bcse.v29i3.12
(19)
Effective Phytomedicine for the Treatment of Benign Prostatic Hyperplasia (BPH) and Ovarian Cyst / Ebenezer Olapade, Ebenezer Olapade Jrn, Clemente Olapade, Christiana Olapade, Ojo and Babajide Olapade /
(20)
Revisiting the taxonomy of Dioclea and related genera (Leguminosae, Papillonoideae), with new generic circumscriptions / Luciano Paganucci de Queiroz, Cristiane Snak / Phytokeys, 164: pp 67-114 /
DOI: https.://doi.org/10.3837/phytokeys.164.55441
(21)
Dioclea reflexa / Ken Fern: Tropical Plants Database / Useful Tropical Plants
(22)
Quantification of the Total Phenolic and Flavonoid Contents,and Antibacterial Activity of Dioclea reflexa / Edewor TI, Akpor OB, Owa SO / International Journal of Pharm Tech Research, Oct-Nov 2014; 6(6): pp 1875-1880 / ISSN: 0974-4304
(23)
Steroids Constituents of Dioclea reflexa Hook Seeds / F J Faleye / Journal of Pharmaceutical and Scientific Innovation, 2012; 1(3): pp 89-90
(24)
Chemical composition and in vitro digestibility of some selected under-utilized tropical s3eeds as protein source in ruminants' diet / Oluwatosin Bode Omotoso, Mary Oluwafunmilayo Adeduntan, Adebowale Noah / Bulletin of the National Research Centre, 2020; 24: Art No 175 / DOI: https://doi.org/10.1186/s42269-020-00430-9
(25)
Formulation and Characterization of Metronidazole Suspension Using Gum Extracted from Dioclea reflexa Seeds / Chukwuemeka C Mbah, Odinaka E Omeje, Sabinus I Ofoefule et al / Pharmaceutical Development and Industrial Pharmacy, June 2020; 2(1)

α(5)

DOI: It is not uncommon for links on studies/sources to change. Copying and pasting the information on the search window or using the DOI (if available) will often redirect to the new link page. (Citing and Using a (DOI) Digital Object Identifier)

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