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Family Verbenaceae
Lagundi
Vitex negundo Linn.
FIVE-LEAF CHASTE TREE
Huang jing zi

Scientific names  Common names
Agnus-castus incisa (Lam.) Carriére Dabtan (If.) 
Agnus-castus negundo (L.) Carriére Dangla (Ilk.) 
Vitex arborea Desf. Kamalan (Tag.)
Vitex chinensis Mill. Lagundi (Ibn., Tag., Bik., P. Bis.)
Vitex elmeri Moldenke Liñgei (Bon.)
Vitex gracilis Salisb. Limo-limo (Ilk.)
Vitex incisa Lam. Sagarai (Bag.)
Vitex laciniata Schauer Turagay (Bis.)
Vitex leucoxylon Blanco [Illegitimate] Chaste tree (Engl.)
Vitex negundo Linn. Chinese chastetree (Engl.)
Vitex sinuata Medik. Cut leaf vitex (Engl.)
Vitex spicata Lour. Five-leaved chaste tree (Engl.) 
Infraspecific taxa  
Vitex negundo var. cannabifolia (Siebold & Zucc) Hand.-Mazz.  
Lagundi is a shared common name by two Vitex species: Vitex negundo and Vitex trifolia.
Vitex negundo L. is an accepted name The Plant List

Other vernacular names
BENGALI: Nishinda, Nirgundi, Samalu.
CHINESE: Huang jing zi, Huang ping, Huang ching, Man ching.
GUJARATI: Nagoda.
HINDI: Nirgundi, Sindvar, Samhalu, Meudi.
KHMER: Trasiet.
MALAYALAM: Vennocchi.
MARATHI: Nirgunda.
NEPALI: Simali.
PUNJAB: Shwari.
SPANISH: Agno-casto.
SANSKRIT: Sinduvara, Sindhuvara, Indrani, Nilanigundi, Nirgundi, Sephalika.
TAMIL: Nirkundi, Noochi, Vellai-nochi.
TELUGU: Vavili.
THAI: Khon thi khamao.
VIETNAMESE: Ngux traro.

Botany
Lagundi is an erect, branched tree or shrub, 2 to 5 meters high. Leaves are usually 5-foliate, rarely with 3 leaflets only, and palmately arranged. Leaflets are lanceolate, entire, 4 to 10 centimeters long, slightly hairy beneath, and pointed at both ends, the middle leaflets being larger than the others, and distinctly stalked. Flowers are numerous, blue to lavender, 6 to 7 millimeters long, borne in terminal inflorescences (panicles) 10 to 20 centimeters long. Calyx is hairy, and 5-toothed. Corolla is densely hairy in the throat, and the middle lobe of the lower lip is longest. Fruit is a succulent drupe, globose, black when ripe, about 4 millimeters in diameter.

Distribution
- Widely distributed in the Philippines.
- At low and medium altitudes, in thickets and waste places.
- Flowering year round. Best propagated by use of mature, leafless stem cuttings.

- Also occurs in tropical East Africa, Madagascar, India to Japan, and southward through Malaya to western Polynesia.

Parts utilized
- Leaves, bark, roots and seeds.
- Leaves may be harvested three months after establishment.

Properties
• Plant is considered anti-inflammatory, astringent, antibacterial, antifungal, analgesic, alterant, depurative, rejuvenating, stomachic.
• Roots considered tonic, febrifuge, anti-rheumatic, diuretic and expectorant.
• Leaves and seeds considered vulnerary.
• Leaves are considered aromatic, bitter, anti-inflammatory, bronchial smooth muscle relaxant, lactagogue, emmenagogue, insecticide, and vermifuge.
• Flowers are astringent, carminative, hepatoprotective, digestive, vermifuge and febrifuge.
• Fruit is considered nervine, cephalic, aphrodisiac, emmenagogue and vermifuge.

Constituents
• Volatile oil; resin; alkaloid; lichen acids; glucoside.
• Constituents of oil: sabinene, linalool, terpinen-4-ol, b-caryophyllene, a-guaine and globulol.
• Study on essential oils showed B-caryophyllene common to leaves, flowers and dried fruits.
• Leaves yield a colorless essential oil and a resin; the fruit yields an acid resin, an astringent organic acid, mallic acid, and coloring matter.
• Leaves contain an alkaloid nishindine, flavones, luteolin-7-glucoside, casticin, iridoid glycosides.
• Phytochemical screening of ethanol leaf extract yielded alkaloids, iridoids, phenolic acids, flavonols and flavonoids.
• Seeds contain hydrocarbons, B-sitosterol, benzoic acid and phthalic acid, anti-inflammatory diterpene, flavonoids and triterpenoids.
• Essential oil of seeds yielded forty-two components representing 91.36% of the oil. Major constituents were n-Hexadecanoic acid (17.68%), eudesm-4(14)-en-11-ol (12.39%) and caryophyllene oxide (10.79%) were found to be the major constituents. (See study below) (43)
• Essential oil analysis fresh leaves, flowers and dried fruit yielded main constituents, viz., leaves: α-guaiene, caryophyllene epoxide and ethyl-hexadecenoate; flowers:-α-selinene, germacren-4-ol, caryophyllene epoxide and (E)-nerolidol; fruit:
β-selinene, α-cedrene, germacrene D and hexadecanoic acid. (See studies below) (46)
• Aerial parts of Vitex negundo var. cannabiolia yielded four phenolics,
salviaplebeiaside (1), γ-tocopherol (2), chrysosplenol-D (4), and isovitexin (5), along with α-tocoquinone (3) and β-sitosterol (6). (see study below) (54)
• Methanolic leaf extract by FTIR spectroscopy yielded alcohols and phenols, alkane, alkene, carboxylic acid, aromatic compound, alipathic nitro compound, primary alcohol, para-benzene, meta-benzene, and bromo alkanes. (66)
• Study on leaves yielded pure compounds of triterpenoidal nature, i.e. oleonolic acid and lupeol for the first time. (71)

Uses
Edibility
- Seeds used as condiment as pepper substitute.
- Leaves and roots used in making tea.
Folkloric
- Decoction of leaves used externally for cleaning ulcers and internally for flatulence. Also used as a lactagogue and emmenagogue.
- Decoction of bark, tops and leaves used as antigastralgic.
- Leaves used in aromatic baths; also as insectifuge.
- Vapor bath prepared with the plant used for treatment of febrile, catarrhal, and rheumatic affections.
- Decoction of leaves used as warm bath by women suffering with after-pains in the puerperal period. Also used as baths for new born children.
- Seeds are boiled in water and eaten or the water drunk to prevent the spread of toxin from bites of poisonous animals.
- Infusion of seeds used for disinfecting wounds and ulcers.
- Infusion of seeds in wine used for dropsy.
- Pounded leaves applies on the forehead and temples for headaches.
- Leaf decoction for fever, headache, toothache, cough, asthma.
- Root used as tonic, febrifuge and expectorant.
- Fruit used as nervine, cephalic, and emmenagogue.
- Tincture of root bark used for irritable bladder and for rheumatism.
- Powdered root used for piles as demulcent; also for dysentery.
- Root used for dyspepsia, colic, rheumatism, worms, boils, and leprosy.
- Flowers are used for diarrhea, cholera, fever, and diseases of the liver; and also as cardiac tonic.
- Powdered flowers and stalks are used for bleeding from the stomach and bowels.
- Fruit used for headaches, catarrh, and watery eyes. Dried fruits are used as vermifuge.
- Seeds are prepared as cooling medicine for skin diseases, leprosy, and inflammation of the mouth.
- Oil prepared with the juice used for sinuses and scrofulous sores. Oil also used as a rubbing application to glandular or tubercular swelling of the neck. Oil also used for treatment of sloughing wounds and ulcers.
- Leaves used for reducing inflammatory and rheumatic swellings of the joints and testicular swelling associated with gonorrheal epididymitis and orchitis. Poultice of leaves also applied to sprained limbs, contusions, leech bites, etc. For these, fresh leaves in an earthen pot are heated over fire, and applied and applied as tolerated over the bruised parts. Leaves heated over fire are also applied with oil externally on wounds.
- Pillow stuffed with leaves is placed under the head for relief of catarrh and headache. Dried leaves when smoked also used to relieve catarrh and headaches.
- Decoction of leaves and long pepper used for catarrhal fever associated with head congestion and dullness of hearing.
- Juice of leaves used to remove fetid discharges and worms from ulcers.
- Plaster of leaves applied to enlarged spleens.
- Folkloric preparations: (1) For fever and toothaches, boil 6 tbsp of the chopped leaves in 2 glasses of water for 15 minutes; strain and cool. Divide the decoction in 3 parts and take one part every 3-4 hours. Also, bruised leaves may be applied to forehead. (2) For asthma and cough: Take 1/4 of the decoction three times a day. (3) Aromatic bath or sponge bathing: Boil 4 handfuls of leaves in a pot of water for 5 minutes; use the lukewarm decoction for sponge bathing.
- In Ayurveda and Unani, leaves and seeds used for rheumatism and joint inflammation. Decoction of leaves taken as a diuretic. Used for pacifying vata nerves.
- In Bangladesh, used for headaches, weakness, vomiting, malaria black fever.
- In Indo-China, root decoction used for intermittent fevers.
- In Sri Lanka, used for eye disease, toothache, rheumatism; used as tonic, carminative and vermifuge.
Others
- Insecticide / Fumigant: Leaves considered insecticide and placed between pages of books and folds of silk and woolen clothing to preserve them from insects. Fresh leaves burnt with grass as fumigant against mosquitoes.
- Dyeing: Ashes much used as alkali in dyeing.
- Basketry: Young stems used for making baskets.
- Stems used for making wattles.
Recent Use
Lagundi has been proven to be an effective analgesic and antitussive (prepared as a pleasant tasting cough syrup) and has been considered as a replacement for dextromethorphan in the public health system.
New Application
Studies have shown benefit through reduction of coughing and relaxation of the bronchial smooth muscles. Being promoted by the Department of Health (DOH) for cough and asthma. One of a few herbs recently registered with the Bureau of Foods and Drugs (BFAD) as medicines.

Preparation
How to make lagundi syrup
• Clean fresh lagundi leaves and chop.
• In 4 glasses of water, boil 4 tablespoons of minced lagundi leaves for 15 minutes.
• Strain the liquid extract and add 1 part honey to 4 parts extracts.
• Boil in an earthen pot or enamel-lined saucepan for 15 minutes until the desired viscosity is attained; cool.
• Pour the syrup in clear amber-colored bottles.
(6)

Studies
Anti-Venom:
Tested against Vipera russellii and Naja kaouthia venom, a methanolic extract study of VN showed it possesses potent snake venom neutralizing capacity and suggests further investigation.   (1)
Anti-Inflammatory / Potentiation of Phenylbutazone and Ibuprofen: Study showed sub-effective dose of VN significantly potentiated anti-inflammatory activity of phenylbutazone and ibuprofen in albino rats in carrageenan induced hind paw edema and cotton pellet granuloma models. (2)
Anti-Inflammatory / Prostaglandin Inhibition: Study suggests VN possess anti-inflammatory activity against acute and sub-acute inflammation probably due to prostaglandin inhibition and reduction of oxidative stress. (3)
Anti-Inflammatory / Analgesic: Study showed the fresh leaves of VN have anti-inflammatory and pain suppressing activities possibly mediated through PG synthesis inhibition, antihistamine, membrane stabilizing and antioxidant activities.
Antibacterial / Essential Oil: Study showed the essential oils and extracts to have antibacterial activity. Essential oil and extracts showed promising results against B subtilis and E coli. Ethyl acetate and ethanol extracts showed prominent antibacterial activity against all tested strains. (4)
Antibacterial / Leaf, Flower and Fruits: Study of extracts of leaf, flower and fruit of VN was done to evaluate in vitro antibacterial activity against phytopathogens Pseudomonas solanacearum and Xanthomonas axonopodis. The ethyl acetate extract showed significant inhibition. Phytochemical screening yielded alkaloids, flavonoids, tannins, cardiac glycosides and terpenoids.
Antifungal: (1) New antifungal flavonoid glycoside from Vitex negundo: Study found a new isolated flavone glycoside and a known compound to have significant antifungal activity against Trichophyton mentagrophytes and Cryptococcus neoformans. (2) Ethanol extract of fruit seeds showed significant activity against Fusarium solani and moderate response against Microsporum canis with no effect against C albicans.
Larvicidal: Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae: The methanolic extracts of all Vitex species showed varying levels of larvicidal activity.
(7)
Anthelmintic: Study of ethanolic extracts of Moringa oleifera and Vitex negundo on anthelmintic activity against Indian earthworm Pheretima posthuma showed both to have dose dependent activity, with Moringa oleifera showing more activity.
(8)
Antioxidant:
Report indicated VN can produce reduction of oxidative stress mainly by reducing lipid peroxidation. (•) Study of ethanolic leaf extract showed antioxidant activity attributed to the presence of phenolic compounds like flavonoids and flavonols. (•)
Study showed the leaves showed 23.21 mg/100 of Ascorbic acid Equivalent Antioxidant Capacity (AEAC). (•) Study of 17 Indian medicinal plants, including the alcoholic extract of VN, all showed dose-dependent nitric oxide (NO) scavenging activity. (11)
Anticonvulsant / Adjuvant Therapy: Study evaluated the anticonvulsant activity of VN leaf extracts in albino mice. Results suggest that VN possesses anticonvulsant activity particularly against PTZ (pentylenetetarazole) induced seizures, with a significant reduction of number and duration of convulsions. The potentiation of diphenylhydantoin and valproic acid suggests it may be useful as adjuvant therapy to lower the requirements of the drug therapies. (23)
Insecticidal / Pesticidal: Studies have shown the plant products to possess insecticidal activity against mosquito larvae, houseflies and stored product pests.
Pharmacokinetic Interaction / V. negundo and Paracetamol: Study showed a significant decline in plasma concentration of paracetamol. Results conclude that if the VN extract or an ayurvedic formulation is co-administered with an allopathic drug like paracetamol, the allopathic drug has to be adjusted for achieve its desired therapeutic response. (13)
Antibacterial / Cytotoxic: Study showed all fractions with prominent zones of inhibition against B subtilis, B megaterium, S typhi, Vibrio mimicus and a fungal strains, A niger. Results also showed significant cytotoxic activity against brine shrimp nayplii. (14)
Antimicrobial: Extracts were tested against five bacterial species (S aureus, P vulgaris, B subtilis, E coli, P aeruginosa) and three fungal species ( A niger, A flavon, C albicans). Among all extracts the water/ethanol extract showed maximum antimicrobial activity and the water extract, maximum antifungal activity against all species tested.
Gastroprotective / Anti-Ulcer / Flavonoids: Study in albino rats investigating the gastroprotective activity of the aqueous extract of VN against aspirin-induced mucosal damage revealed VN to have a pivotal role in treating ulcer. Phytochemical studies yielded the presence of flavonoids probably responsible for its gastroprotection.
Hepatoprotective / Negundoside: Negundoside, an iridoid glycoside from the leaves of VN was studied for its hepatoprotective effect on CCl4-induced liver toxicity. Results showed NG exerts a protective effect of CYP2E1-dependent CCl4 toxicity via inhibition of lipid peroxidation, followed by improved intracellular calcium homeostasis and inhibition of Ca-dependent proteases.
(15)
Anxiolytic: Study showed VN is an effective anxiolytic agent. The action of the extract upon anxiety models tested were consistent with the traditional use of VN and presents a potential for use in primary medical care.
(16)
Essential Oil / Flowers: Study on the essential oil of flowers of VN yielded 45 components. The major compounds were sabinene (20.3%), B-caryophyllene (14.1%) and globulol (19.2%). (19)
Antinociceptive / Anti-Inflammatory / Seeds: Study showed ethanol extract of VN seeds interacted with the opioid system and may be more effective3 on inflammatory pain. Further results suggest that the analgesic effects may be partially mediated by it anti-inflammatory activity. The analgesic activity could be due to the abundance of fatty acids with synergistic effects.
(20)
Anti-Microfilarial: Study investigated the antifilarial effect of V. negundo against Brugia malayi microfilariae. A root extract of VN caused complete loss of motility of microfilariae after 48 hrs of incubation. Study yielded the presence of alkaloids, saponins and flavanoids from the roots of VN.
Antinociceptive / Leaves: Study in mice investigating the antinociceptive activity of an ethanolic leaf extract showed significant dose-dependent analgesic activity. Ten times the extract dose produced the effects comparable to the standard drug meperidine. Naloxone did not reverse the analgesic effect of the VN extract. Results suggest both central and peripheral analgesic activity and also suggests a potential as adjuvant therapy with analgesic drugs.
(24)
Antiamnesic: Study investigated the anti-amnesic activity of VN in scopolamine-induced amnesia in rats. Results showed that VN treated groups had decreased phenomenon of amnesia by increasing learning of memory through antioxidant effect and decreasing AChE activity.
(27)
Cytotoxicity / Antitumor: Study of ethanol and aqueous extract of leaves of Vitex negundo against Dalton's Ascitic Lymphoma showed antitumor effect. (29) Study of showed a hydroalcoholic extract of aerial parts showed higher in vitro cytotoxicity activity against Dalton's ascites lymphoma line. (48)
Hepatoprotective / Paracetamol-Induced Injury: Study of ethanolic extract showed V. negundo was effective in protecting the liver against paracetamol-induced injury in rats. (31)
Antioxidant / Antiproliferative / Pass-Predicted V. negundo: VN extract showed the strongest free radical scavenging power compared to two commercial antioxidants. An ethanolic extract showed cytotoxicity to HepG2 cells in a dose- and time- dependent manner. The experimental studies verified the predictions obtained by a PASS-predicted design strategy. (35)
Amelioration of Induced Colitis / Leaves: An ethanolic extract of leaves of V. negundo showed significant amelioration of experimentally induced colitis, which may be attributed to its anti-inflammatory and antioxidant property. (36)
Larvicidal / Mosquitocidal: Study showed V. negundo ethanol leaf extracts had larvicidal activity against mosquito larvae. (37) Study evaluated crude leaf extracts of Leucas aspera, Vitex negundo and Eucalyptus for larvicidal activity against Culex quinquefasciatus. Eucalyptus and V. negundo showed good larvicidal activity against Cx. quinquefasciatus. Leucas aspera showed poor mortality. Four different solvent extracts of V. negundo showed good larvicidal activity. Results suggest a potential for the crude extracts for mosquito control and replacement of chemical pesticides. (52)
Antidiabetic Potential / Leaves: Study evaluated aqueous and ethanol leaf extracts of V. negundo for antidiabetic activity in alloxan induced diabetic rat models. Results showed the aqueous extract with significant activity, greater than the ethanol extract, and comparable to glibenclamide, the standard antidiabetic drug. (38) Study of crude ethanolic extracts of V. negundo leaves extract showed significant hypoglycemic and hypolipidemic activity against streptozotocin-induced diabetic mice. (61)
Dermal Toxicity Study / Essential Oil: Study evaluated V. negundo essential oil for potential acute and subchronic dermal toxicities in Wistar rats for five weeks. Results showed all animals to be normal without any behavioral, chemical, hematological, necroscopical and histopathological changes, with NOEL (no observed effect level) and NOAEL (no observed adverse effect level) on 250 to 1000 mg/kbw/day, respectively. (39)
HIV-1 Reverse Transcriptase Inhibition / Leaves: Study evaluated the effects f an ethanolic leaf extract of Vitex negundo against HIV-1 Reverse Transcriptase (RT) and to identify and quantify the flavonoids present. Results showed VN possess anti-RT substances. The activity was attributed possibly to the presence of flavonoids, in particular, the high quantity of kaempferol, myricetin, and quercetin. (40)
α-Amylase / Lowering of Post Prandial Hyperglycemia: Study evaluated the alpha amylase inhibitory effects of flavanoid extracts of different part of V. negundo and A. paniculata. Except for Vitex leaf flavonoid extract, all other tested flavonoids of both plant parts showed ore than 50% inhibition of α-amylase activity, indicating the flavonoids of both plants may be effective in lowering post prandial hyperglycemia. (41)
Miticidal / Anti- Scabies: Study evaluated the miticidal effect of a methanolic extract of V. negundo through topical applications on scabies-affected camel, buffalo, goat, dog, and man. Results showed 10, 20, and 30% concentrations to cause 70, 80, and 90% mortality of Sarcoptes scabei mites, compared to ivermectin (85%) and methyl alcohol (5%) mortality. (42)
Antifungal / Essential Oil: Study investigated the constituents and antimicrobial activity of essential oil from V. negundo seeds. Results yielded forty-two components, and the oil exhibited significant antifungal activity against Candida albicans. (43)
Anxiolytic / Essential Oil: Study evaluated the efficacy of ethanolic extracts of leaves of V. negundo in animal tests of anxiety. In mice using the elevated plus maze test, results showed anxiolytic behavior similar to diazepam. (44)
Antieosinophilic / Anti-Asthma / Leaves: Study evaluated leaf extracts and fractions for action on bronchial hyperresponsiveness using egg-albumin induced asthma in guinea pigs. Results showed the aqueous subfraction of leaves possessed antieosinophilic activity, reducing bronchial hyperresponsive-ness. Results suggest potential usefulness in the treatment of asthma and various inflammatory, allergy, and immunologic disease. (45)
Antibacterial / Essential Oils: Essential oils from fresh leaves, flowers, and fried fruits were evaluated for antibacterial potential against S. aureus, B. subtilis, E. coli, and P. aeruginosa. All the essential oils and successive extracts showed activity against B. subtilis and E. coli. EA and ethanol extracts showed activity against all tested strains. Fruits and leaves were most active against E. coli and S. aureus. Only the flower oil was active against P. aeruginosa. (see constituents above) (46)
Toxicity Studies / Essential Oils: Study evaluated combined extracts of V. negundo, V. leucoxylon, and V. trifolia for toxicity in mice. Results showed no toxicity or evidence of adverse effects in mice following acute oral administration at highest dose of 2000 mg/kg crude extracts. (47)
Neuroprotective / Ethanol Induced Cerebral Oxidative Stress: Study evaluated various fractions of hydromethanolic extract of leaves against ethanol-induced cerebral oxidative stress in rats. Results showed protective action on the brain, attributed to its antioxidant potential. The chloroform fraction activity was comparable to standard α-tocopherol. (49)
Anti-Typhoid Activity / Leaves: Study evaluated the methanolic leaf extracts of V. negundo and A. vasica for anti-typhoid activity against Salmonella typhi. Leaf extracts of both V. negundo and V. vasica showed considerable antioxidant activity and anti-typhoid activity. (50)
Anti-Inflammatory / Leaves: Study evaluated Vitex negundo, A. marmelos and B. serrata for potential anti-inflammatory activity in LPS treated human lung adenocarcinoma A549 cells. The methanolic extract of all 3 plants significantly decreased the LPS induced NO production and pro-inflammatory cytokines expression. Of the 3, Vitex negundo and Aegle marmelos leaf extracts showed potent anti-inflammatory activity at 50 and 100 µg/mL. (53)
Antiseptic / Aerial Parts: Study yielded four phenolics (1-4), along with a-tocoquinone and ß-sitosterol from aerial parts of V. negundo var. cannabifolia. Compound 4, chrysosplenol-D exhibited antibacterial activities against four spoilage test microorganisms viz. E. coli, B. subtilis, Micrococcus tetragenus, and Pseudomonas fluorescens. (see constituents above) (54)
• Antibacterial against Multidrug Resistant Bacterium: Study investigated the antibacterial activity of extracts and essential oils of V. negundo leaves against an unknown bacterium resistant to various antibiotics. The ethanol extracts of V. negundo showed ability to inhibit growth or kill concerned multidrug resistant bacterium (B. cereus strain mmm86) and can be used for pharmaceutical purposes The activity may be due to presence of some compounds like ß-caryophyllene, Ag (silver) nanoparticles, betulinic acid, etc. (55)
• Anti-Microfilarial / Leaves: Study evaluated the possible antifilarial effect of an ethyl acetate extract of Vitex negundo leaves against Setaria cervi filarial parasite in vitro. Complete inhibition of motility was observed in motility assay. Antifilarial effect was found to be a function of relative concentrations. Inhibitory concentration (IC50) was 0.16 mg/ml. (56)
• Antivenom / Antioxidant / Leaves: Study investigated a hydroethanolic extract of V. negundo leaves (blue and green) for antioxidant, antiplatelet, antihemolytic, and in-vitro antivenom potential against Naja naja and Daboia russelii venoms. Results showed the blue leaf extract of V. negundo exerted potent antioxidant and venom neutralizaing effect compared with the green leaf extract. (57)
• Anti-Inflammatory / Roots: Study evaluated the anti-inflammatory effect of an ethanolic extract of Vitex negundo roots in rats. Results showed considerable (p<0.05) anti-inflammatory effect in a carrageenan-induced rat paw edema model. (58)
• Inflammatory Bowel Disease / Leaves: Study of ethanolic extract of leaves of Vitex negundo showed significant amelioration of experimentally induced inflammatory bowel disease (IBD) in albino rats. Activity was attributed to its anti-inflammatory and antioxidant property. (59)
• Acute Toxicity Study / Leaves: Acute oral toxicity evaluation of ethanolic extract of leaves of V. negundo was done using OECD guidelines. Results showed safety even at doses more than 2000 mg/kg without any sign of toxicity or mortality. (59) Preliminary acute toxicity study of ethanolic leaf extract in albino rats by oral route showed it to bee practically nontoxic with an LD50 of 7.5 g/kbw. (Tandon and Gupta, 2003) (65)
α-Chymotrypsin Inhibition of Vitex negundo Lignans / Roots: Lignans isolated from the roots of Vitex negundo were screened against serine proteases α-chymotrypsin, thombin, and prolyl endopeptidase. Compounds 3 and 4 were active only against
α-chymotrypsin as competitive and non-competitive inhibitors of the enzyme, respectively. (60)
• Hepatoprotective / Leaves: Study evaluated the hepatoprotective activity of ethanolic extract of leaves of Vitex negundo against thioacetamide (TAA)-induced hepatic injury in Sprague Dawley rats. Results showed hepatoprotective effect as evidenced by intervention in the progression of liver fibrosis induced by TAA in rats. (62)
• Antiulcer / Leaves: Study evaluated the antiulcer activity of ethanolic extract of V. negundo leaves in pylorus ligation and aspirin induced gastric ulcer models in rats. In the pylorus ligation model, study showed reduced ulcer index (1.66), decrease in total gastric acid and free acid (p<0.0001), increase in pH value (p<0.0001), and reduced gastric volume (p<0.0003), increase percentage of ulcer protection (61.66%). In the aspirin induced model, there was decrease in ulcer index (10.66) and increase in ulcer protection (72.09%). (63)
• Effect on Surgically Induced Endometriosis / Leaves: Study evaluated the effect of V. negundo aqueous leaf extract on surgically induced endometriosis in Sprague Dawley rats. Results showed reduction in endometriosis cyst size, adhesion, histological grading, and oxidant levels as well as elevation in antioxidant level. Activity may be associated with multiple synergistic mechanisms due to the presence of various phytochemicals such as carbohydrate, coumarins, flavonoids, phenol, resins, saponins, tannin, and terpenoid. (64)
• Antidiarrheal / Antispasmodic: Study evaluated the pharmacologic basis of its medicinal use in hyperactive gut disorders. A crude extract showed dose-dependent protection (53-71%) in a castor oil-induced diarrhea model, similar to loperamide. In isolated rabbit jejunum preparation, there V. negundo showed inhibition of spontaneous and high K+-induced contractions. Data results suggest that the antidiarrheal and spasmolytic effects of crude extract may be mediated through the presence of CCB (calcium channel blockade)-like constituent/s. (67)
• Testosterone Lowering Potential in Induced PCOS / Seeds: Study evaluated the testosterone lowering potential of Vitex negundo seed extract in hyperandrogenised female Sprague Dawley rats induced with PCOS (polycystic ovarian syndrome). Results showed significant reduction of serum testosterone and serum glucose. Histopathology showed less follicular cysts and lesions. Study showed promising potential as an anti-androgen which may be due to phytosterols and flavonoid contents. (68)
• Hepatoprotective / Acetaminophen and Galactosamine Hepatotoxicity: Study evaluated the liver protective efficacy of a standardized bioactive fraction (SF) from V. negundo against acetaminophen (APAP) and galactosamine (GaIN) hepatotoxicity. Results showed significant hepatoprotective activity attributed to its antioxidant mechanism as evidenced by protection against increased lipid peroxidation and maintained glutathione status. Study showed agnuside and negundoside were active ingredients i the standardized fraction. (69)
• Herbal Bath Soap / Antibacterial and Antifungal / Leaves: Study evaluated the formulation of an herbal bath soap using V. negundo leaf extract. Results showed the formulation to be a stable solid and categorized as Grade 2 soap. The antibacterial and antifungal activities of the formulated soap were significantly higher than commercial antibacterial and antifungal soaps. (70)

Availability
- Wild-crafted.
- Commercial formulations: Tablets, capsules, oil, teas, and syrup. 

© Godofredo U. Stuart Jr., M.D.

Updated September 2017 / September 2016

Photos © Godofredo Stuart / StuartXchange
IMAGE SOURCE / Public Domain / File:Vitex negundo Blanco1.228-cropped.jpg / Flora de Filipinas / Franciso Manuel Blanco (OSA), 1880-1883 / Modifications by Carol Spears / Wikimedia Commons

Additional Sources and Suggested Readings
(1)
Snake venom neutralization by Indian medicinal plants (Vitex negundo and Emblica officinalis) root extracts / M I Alam and A Gomes /
Journal of Ethnopharmacology, Vol 86, Issue 1, May 2003, Pages 75-80 / doi:10.1016/S0378-8741(03)00049-7
(2)
Vitex negundo Linn (VN) leaf extract as an adjuvant therapy to standard anti-inflammatory drugs / Vishal R. Tandon & R.K. Gupta* / Indian J Med Res 124, October 2006, pp 447-450
(3)
Anti-inflammatory Activity and Mechanism of Action of Vitex negundo Linn
/ Vishal Ramprakash Tandon, Rajesh Kumar Gupta / International Journal of Pharmacology 01/2006;
(4)
Essential oil composition and antibacterial studies of Vitex negundo linn. extracts / S L Khokra, O Prakash et al / Indian J Pharm Sci. 2008 Jul–Aug; 70(4): 522–526. / doi: 10.4103/0250-474X.44610.
(5)
New antifungal flavonoid glycoside from Vitex negundo
(6)

Lagundi Syrup Herbs and Spices by Ernesta G. Quintana . Negosiete Plus. July 1993
(7)

Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae
(8)
Comparative Studies on Anthelmintic Activity of Moringa Oleifera and Vitex Negundo / Trapti Rastogi et al / Asian Journ Research Chem. 2(2):April-June, 2009 /
(9)
ANTIFUNGAL ACTIVITIES OF VITEX NEGUNDO LINN. / Shaukat Mahmud et al / Pak. J. Bot., 41(4):1941-1943, 2009 /

(10)
Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo / M G Dharmasiri, JRAC Jayakody et al / Journal of Ethnopharmacology, Vol 87, Issues 2-3, August 2003, Pages 199-206 /
doi:10.1016/S0378-8741(03)00159-4
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The Evaluation of Nitric Oxide Scavenging Activity of Certain Indian Medicinal Plants In Vitro: A Preliminary Study / Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga. Journal of Medicinal Food. Fall 2004, 7(3): 343-348. doi:10.1089/jmf.2004.7.343.
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Medicinal Uses and Biologic Activities of Vitex negundo / Vishal R Tandon / Review Article / Natural Product Radiance
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Pharmacokinetic-interaction of Vitex negundo Linn. & paracetamol / Yamini Bhusan Tripathi, Om Prakash Tiwari et al / Indian J Med Res 130, October 2009, pp 479-483
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Antibacterial and cytotoxic activity screening of leaf extracts of Vitex negundo (Fam: Verbenaceae) /
Chowdhury J.A. et al /J. Pharm. Sci. & Res. Vol.1(4), 2009, 103-108.
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Negundoside, an iridiod glycoside from leaves of Vitex negundo , protects human liver cells against
calcium-mediated toxicity induced by carbon tetrachloride
/ Sheikh A Tasjuq, Peerzada J Kaiser et al / World J Gastroenterol 2008 June 21; 14(23): 3693-3709 / doi:10.3748/wjg.14.3693
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Anxiolytic activity of Vitex negundo Linn. in experimental models of anxiety in mice / R S Adnaik, P T Pai et al / Int J Green Pharm, 2009;Vol 3, Issue 3, pp 243-7.
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In-Vitro Evaluation of Anti-Bacterial and Anti-Fungal Activity of Vitex nigundo (Verbenaceae)
/ P B Aswar et al / Ethnobotanical Leaflets 13: 962- 67, 2009.
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Effect of Vitex negundo on oxidative stress / V Tandon, R K Gupta / Indian J Pharmacol | February 2005 | Vol 37 | Issue 1 | 37-45
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Essential Oil Composition of Vitex negundo L. Flowers / Pran N Kaul, B Rao et al / Journal of Essential Oil Research: JEOR, Sep/Oct 2005
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Antinociceptive activities of the liposoluble fraction from Vitex negundo seeds / Cheng-Jian Zheng et al / Summary Pharmaceutical Biology, June 2010, Vol. 48, No. 6, Pages 651-658 / DOI 10.3109/13880200903241838
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Anti-microfilarial activity of methanolic extract of VN and AM and their phytochemical analysis / K N Sahare / Indian Journal of Experimental Biology, Vol 46, Feb 2008, pp 128-131
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Sorting Vitex names / MULTILINGUAL MULTISCRIPT PLANT NAME DATABASE
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AN EXPERIMENTAL EVALUATION OF ANTICONVULSANT ACTIVITY OF VITEX-NEGUNDO / V R Tandon and R K Gupta / Indian J Physiol Pharmacol 2005; 49 (2) : 199–205
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ANTINOCICEPTIVE ACTIVITY OF VITEX-NEGUNDO LINN LEAF EXTRACT / R K Gupta and V R Tandon / Indian J Physiol Pharmacol 2005; 49 (2) : 163–170
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In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts / Raghavendra H. Lakshmanashetty, Vijayananda B. Nagaraj et al / Chiang Mai J. Sci. 2010; 37(3) : 489-497
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Inhibitory Effect of Different Solvent Extracts of Vitex negundo L. and Allium sativum L. on Phytopathogenic Bacteria / E.C.Jeyaseelan, M.K.Pathmanathan, and J.P.Jeyadevan / Archives of Applied Science Research, 2011, 3 (1):1-8
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Anti-Amnesic Activity of Vitex Negundo in Scopolamine Induced Amnesia in Rats / Abhinav Kanwal, Jogender Mehla, Madhusudana Kuncha et al / Pharmacology and Pharmacy, Vol.1 No.1, July 2010
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Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo
/ P. Praveen Kumar, S. Kumaravel and C. Lalitha / African Journal of Biochemistry Research Vol. 4(7), pp. 191-195, July 2010
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Antitumor Activity of Vitex negundo Linn. against Dalton's Ascitic Lymphoma / Dewade D R, Christiana A J M et al / International Journal of PharmTech Research, Vol.2, No.2, pp 1101-1104, April-June 2010
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A Review on Vitex negundo L. – A Medicinally Important Plan
t / A S Vishwanathan and R Basavaraju / E
JBS 3 (1) ● April-June 2010: 30-42

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HEPATOPROTECTIVE ACTIVITY OF VITEX NEGUNDO LINN. BY PARACETAMOL INDUCED HEPATOTOXICITY IN RATS / P. Ladda, C. S. Magdum, N. S. Naikwade / International Journal of Pharmacological Research, Vol 1, No 1 (2011) / doi: 10.7439/ijpr.v1i1.148
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Lamiaceae Vitex / Plants for Use
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Sorting Vitex names / /Maintained by: Michel H. Porcher / MULTILINGUAL MULTISCRIPT PLANT NAME DATABASE / Copyright © 1995 - 2020 / A Work in Progress. School of Agriculture and Food Systems. Faculty of Land & Food Resources. The University of Melbourne. Australia.
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Chaste Tree / Common names / Flowers of India
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PASS-predicted Vitex negundo activity: antioxidant and antiproliferative properties on human hepatoma cells-an in vitro study / Farkaad A Kadir, Normadiah M Kassim, Mahmood A Abdulla and Wageeh A Yehye /
BMC Complementary and Alternative Medicine 2013, 13:343 doi:10.1186/1472-6882-13-343
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EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF Vitex negundo L. ON ACETIC ACID INDUCED COLITIS IN ALBINO RATS. / SWARNAMONI DAS*, LALIT KANODIA / Asian J Pharm Clin Res, Vol 6, Issue 3, 2013, 138-141

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LARVICIDAL ACTIVITY OF Vitex negundo Linn. (LAGUNDI) AGAINST MOSQUITO LARVAE
/ A Research Paper, March 2013 / CHESKA MARI B. GANHINHIN ARA PATRICE M. KINTANAR / Department of Science and Technology PHILIPPINE SCIENCE HIGH SCHOOL - CENTRAL VISAYAS CAMPUS Talaytay, Argao, Cebu
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Antidiabetic Potential of Aqueous and Ethanol Leaf Extracts of Vitex negundo /
Prasanna Raja.P, Sivakumar. V , Riyazullah. M. S. / International Journal of Pharmacognosy and Phytochemical Research 2012; 4(2);38-40
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Acute and subchronic dermal toxicity of Vitex negundo essential oil / Pronobesh Chattopadhyay, Subham Banerjee, Manash Pratim Pathak, Amit Agnihotri, Sanjeev Karmakar, Danswrang Goyary, Sunil Dhiman, and Vijay Veer / Cutaneous and Ocular Toxicology / (doi:10.3109/15569527.2013.791829)
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HIV-1 reverse transcriptase inhibition by Vitex negundo L. leaf extract and quantification of flavonoids in relation to anti-HIV activity / Mohan KANNAN, Paramasivam RAJENDRAN, V eerasami VEDHA, Gnanasekaran ASHOK, Shanmugam ANUSHKA, Pratap CHANDRAN RAMACHANDRAN NAIR / Journal of Cell and Molecular Biology 10(2):53-59, 2012
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Comparative study of alpha amylase inhibitory activity of flavonoids of Vitex negundo Linn. and Andrographis paniculata Nees / Keerti Gautam, Padma Kumar, Chitra Jain / Int J Green Pharm 2013;7:25-8
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MITICIDAL ACTIVITY OF METHANOLIC EXTRACT OF VITEX NEGUNDO-LAM AGAINST SARCOPTES SCABIEI IN ANIMALS AND MAN / M. A. Khan, A. H. Shah, A. Maqbol*, N. Khan** and Z. U. Rahman / J Anim Plant Sci, 22(Sup 2): 102-107
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Antifungal Properties and Chemical Analysis of Essential Oil from Vitex negundo Seeds / Hong-Wei Ai, Yue-Xin Kang, Yang Cao and Cheng-Jian Zheng* / British Journal of Pharmaceutical Research, ISSN: 2231-2919,Vol 4, No 5 (May)
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Anxiolytic like effects of leaves extract of Vitex negundo (L) (fam:-verbaceae) in elevated plus maze test
/ Manoj K. Aswar*, Abhijeet A. Bidkar, Kishore N. Gujar, * Tanay G. Athawale / Journal of Natural Remedies, Vol. 12/2 (2012) 141-150
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Antieosinophilic activity of various subfractions of leaves of Vitex negundo
/ Jignesh I Patel, Shrikalp S Deshpande / Int J Nutr Pharmacol Neurol Dis 2013;3:135-41 / DOI: 10.4103/2231-0738.112839
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Essential Oil Composition and Antibacterial Studies of Vitex negundo Linn. Extracts / S. L. Khokra,* O. Prakash, S. Jain, K. R. Aneja, and Yogita Dhingra / Indian J Pharm Sci. 2008 Jul-Aug; 70(4): 522–526. / doi: 10.4103/0250-474X.44610
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TOXICITY STUDIES OF COMBINED EXTRACTS OF VITEX LEUCOXYLON, VITEX NEGUNDO AND VITEX TRIFOLIA / K.Phani and A.Ravi Kumar / Journal of Chemical and Pharmaceutical Sciences, Jan-March 2014, Vol 7, Issue 1.
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Preliminary Phytochemical Studies and Invitro Cytotoxic Activies on Vitex negundo (L.) / Vijy Amirtharaj.R*, Mohammed Reyaz.A, Arun Kumar.J, Kaarthikeyan.M, Saivishwathdindu, Dr.N.Senthil Kumar / International Journal of Research in Pharmaceutical and Biomedical Sciences, Vol. 2(4) Oct - Dec 2011
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Protective effect of the leaves of Vitex negundo against ethanol-induced cerebral oxidative stress in rats /
MUTHUSWAMY UMAMAHESWARI*, KUPPUSAMY ASOKKUMAR, NANDAGOPI UMAMAGESWARI, THIRUMALAISAMY SIVASHANMUGAM and VARADHARAJAN SUBHADRADEVI / Tanzania Journal of Health Research, Volume 14, Number 1, January 2012 / DOI: http://dx.doi.org/10.4314/thrb.v14i1.5
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Anti-typhoid Activity of Adhatoda vasica and Vitex negundo / Manoj Kumar*, Sukumar Dandapat, Amit Kumar and M. P. Sinha / Persian Gulf Crop Protection, 2(3): 64-75
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Vitex negundo / Synonyms / The Plant List
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STUDIES ON LARVICIDAL ACTIVITY OF LEUCAS ASPERA, VITEX NEGUNDO AND EUCALYPTUS AGAINST CULEX QUINQUEFASCIATUS COLLECTED FROM COOVUM RIVER OF CHENNAI, INDIA / V.KARTHIKEYAN*, K. SIVAKUMAR, AISHWARYA GOKULDASS AND S. MOHANASUNDARAM / Asian Journal of Pharmaceutical and Clinical Research Vol 5, Suppl 3, 2012
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Anti-inflammatory activity of Vitex negundo, Boswellia serrata and Aegle marmelos leaf extracts in LPS treated A549 cells / Rajeshwari H. Patil, Naveen Kumar M, Babu R. L, and S. Chidananda Sharma* / International Journal of Phytopharmacy Vol. 5 (2), pp.12-20, Mar-Apr 2015 / Journal DOI: 10.7439/ijpp
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Antiseptic Activity and Phenolic Constituents of the Aerial Parts of Vitex negundo var. cannabifolia
/ Tie-Jun Ling, Wei-Wei Ling, Yuan-Jun Chen, Xiao-Chun Wan, Tao Xia, Xian-Feng Du and Zheng-Zhu Zhang / Molecules 2010, 15, 8469-8477; doi:10.3390/molecules15118469
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Antibacterial Activity of Vitex negundo L. against a Multidrug Resistant Pathogenic Bacterium / Md. Mahfuz-Al-Mamun, Md. Monirul Islam, Rejoana Afrin Neera, Md. Rashed Nejum and G. M. Sala Uddin / European Journal of Medicinal Plants, 8(2): 97-106, 2015
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Antifilarial activity of ethyl acetate extract of Vitex negundo leaves in vitro / K N Sahare, V Singh / Asian Pacific Journal of Tropical Medicine, Vol 6, Issue 9, September 2013, Pages 689-692 / https://doi.org/10.1016/S1995-7645(13)60119-4
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In vitro antivenom and antioxidant potential of Vitex negundo leaves (green and blue) against Russell’s viper (Daboia russelli ) and Indian cobra (Naja naja) venom / Brindha Durairaj, Santhoshkumar Muthu and Krupa Shreedhar / Pelagia Research Library European Journal of Experimental Biology, 2014, 4(4):207-219
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Anti-inflammatory activity of ethanolic extract of Vitex negundo linn roots / Atul Kumar Gangwar, Ashoke K. Ghosh, Vikas Saxena / International Journal of Herbal Medicine 2015; 2 (6): 01-02
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EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF Vitex negundo L. ON ACETIC ACID INDUCED COLITIS IN ALBINO RATS. / SWARNAMONI DAS*, LALIT KANODIA / Asian Journal of Pharmaceutical and Clinical Research, Vol 6, Issue 3, 2013
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α-Chymotrypsin Inhibition Studies on the Lignans from Vitex negundo Linn / Muhammad Arif Lodhi, Azhar-ul-Haq et al / Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 23, Issue 3 (2008) / http://dx.doi.org/10.1080/14756360701584653
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Antioxidant and Antidiabetic Properties of Vitex nigundo L. Leaves / Most. Fatimatuz Zahura Falguni, Md. Amirul Islam, Md. Mahmudul Hasan, S. M. M. Mahmud Hossain Mousum, Md. Ashraduzzaman, Shahanaz Khatun* / American Journal of Life Sciences, 2017; 5(1): 21-26 / doi: 10.11648/j.ajls.20170501.14
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Hepatoprotective Role of Ethanolic Extract of Vitex negundo in Thioacetamide-Induced Liver Fibrosis in Male Rats / Farkaad A Kadir, Normadiah M Kassim, Mahmood A Abdulla, and Wageeh A Yehye / Evidence-Based Complementary and Alternative Medicine, Volume 2013 (2013) /
http://dx.doi.org/10.1155/2013/739850
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EVALUATION OF ANTIULCER ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF VITEX NEGUNDO ON PYLORUS LIGATURE INDUCED AND ASPIRIN INDUCED ULCER IN ALBINO RATS / YAKAIAH VANGOORI, DEEPIKA KLNRR, MEGHAVANI GADEKAL, RAMESH P / International Journal of Pharmacy and Pharmaceutical Sciences, Vol 5, Issue 3 (2013)
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Effect of Notchi kudineer (Vitex negundo aqueous extract) on surgically induced Endometriosis in Sprague Dawley rats / Arul Amuthan, Shabari Anandh, Syed Musharraf, Nupur Agarwal, Vasudha Devi, Revathi P Shenoy, Bhavna Nayal / International Journal of Pharmacology and Clinical Sciences, Sep 2015, Vol 4, Issue 3, pp 35-43
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Vitex negundo: Medicinal Values, Biological Activities,Toxicity Studies and Phytopharmacological Actions / Kambham Venkateswarlu / Int.J.Pharm.Phytopharmacol.Res. 2012, 2(2): 126-133
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Evaluation of Phytochemical Compounds in Leaf Extract of Vitex Negundo L. Using TLC, UV-VIS and FTIR Analysis / M.Janakiraman, K.Jeyaprakash / International Journal of Health Sciences & Research, Vol 5, Issue 8; August 2015
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Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade / Munasib Khan, Abdul Jabbar Shah, Anwar Hassan Gilani / Bangladesh Journal of Pharmacology, Vol 8, No 3 (2013) / DOI: http://dx.doi.org/10.3329/bjp.v8i3.15296
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Studying the Testosterone Lowering Potential of Vitex negundo (Nirgundi) Seed Extract in Hyperandrogenic Female Rats Induced with Polycystic Ovaries / Shetty Divya K*, Patil Swati S / Int. J. Pharm. Sci. Rev. Res., 33(1), July – August 2015; Pp 211-216
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Protective Effect of a Standardized Fraction from Vitex negundo Linn. Against Acetaminophen and Galactosamine Induced Hepatotoxicity in Rodents / Neelam Sharmaa, Jyotsna Suric, BK Chandana, B Singha, Naresh Sattib, A Prabhakar, BD Gupta, Mowkshi Khullara and Zabeer Ahmeda / Biochem Anal Biochem 5: 267. / doi:10.4172/2161-1009.1000267
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Formulation of Herbal Bath Soap from Vitex negundo Leaf Extract / Kandasamy Ruckmani*, Rengasamy Krishnamoorthy, Subramani Samuel, Henry Linda Jeeva Kumari / Journal of Chemical and Pharmaceutical Sciences / National Conference on Plant Metabolomics (Phytodrugs – 2014)
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Isolation and characterization of oleonolic acid and lupeol from Vitex negundo leaves / JA Sawale, JR Patel and ML Kori / Journal of Pharmacognosy and Phytochemistry 2017; 6(4): 936-938















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